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STK3/STK4 Rabbit pAb

Anti-STK3/STK4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220426

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220426
Product Name:   Anti-STK3/STK4 Rabbit Polyclonal Antibody
Synonyms:   KRS1; MST2/KRS2; MST1; YSK3; TIIAC
UNIPROT ID:   Q13043 (Gene Accession – NP_006273 )
Background:    Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades. Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily. The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily. Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation.
Immunogen:    Synthetic peptide of human STK3/STK4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human Lymphoma tissue using 220426(STK3/STK4 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human Lymphoma tissue is first treated with the synthetic peptide and then with 220426(Anti-STK3/STK4 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220426(Anti-STK3/STK4 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261514(Anti-STK3/STK4 Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: PC3 cells, hela cells, human bladder carcinoma tissue, A172 cells, hepG2 cells;
Primary antibody: 220426(STK3/STK4 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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