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SYT4 Rabbit pAb

Anti-SYT4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216807

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216807
Product Name:   Anti-SYT4 Rabbit Polyclonal Antibody
Synonyms:   HsT1192
UNIPROT ID:   Q9H2B2 (Gene Accession – BC036538 )
Background:    Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions. Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons. Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus. The proximity of the Synaptotagmin IV gene to markers of several psychiatric disorders suggest an involvement of synaptotagmin IV in human disease. Synaptotagmin V is a dense-core vesicle-specific protein that regulates a specific type of calcium-regulated secretion. Synaptotagmin VI interacts with adaptor protein-2 in a calcium-independent manner. Synaptotagmin VII is widely expressed in non-neuronal tissues.
Immunogen:    Fusion protein of human SYT4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216807(SYT4 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216807(Anti-SYT4 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, Jurkat cells;
Primary antibody: 216807(SYT4 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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