Email: info@neweastbio.com   |  Call: 6109452007

TIGAR Rabbit pAb

Anti-TIGAR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220237

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220237
Product Name:   Anti-TIGAR Rabbit Polyclonal Antibody
Synonyms:   FR2BP; C12orf5
UNIPROT ID:   Q9NQ88 (Gene Accession – NP_065108 )
Background:    This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis.
Immunogen:    Synthetic peptide of human TIGAR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220237(TIGAR Antibody) at a dilution of 1/80(Nucleus,Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220237(Anti-TIGAR Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 220237(Anti-TIGAR Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D261180(Anti-TIGAR Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human normal liver tissue;
Primary antibody: 220237(TIGAR Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications