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TLE1 Rabbit pAb

Anti-TLE1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218779

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218779
Product Name:   Anti-TLE1 Rabbit Polyclonal Antibody
Synonyms:   ESG; ESG1; GRG1
UNIPROT ID:   Q04724 (Gene Accession – BC015747 )
Background:    Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Immunogen:    Fusion protein of human TLE1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218779(TLE1 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218779(Anti-TLE1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 218779(Anti-TLE1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D225162(Anti-TLE1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Jurkat, 231, A549 and A172 cell lysates;
Primary antibody: 218779(TLE1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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