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TM7SF2 Rabbit pAb

Anti-TM7SF2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221029

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221029
Product Name:   Anti-TM7SF2 Rabbit Polyclonal Antibody
Synonyms:   ANG1; NET47; DHCR14A
UNIPROT ID:   O76062 (Gene Accession – NP_003264 )
Background:    Transmembrane 7 superfamily member 2 (TM7SF2, Sterol C14-reductase, 3beta-hydroxysterol Delta-reductase) is a 418 amino acid gene product that belongs to the ERG4/ERG24 family. TM7SF2 is a seven pass transmembrane protein that can localize to the membrane of the endoplasmic reticulum. TM7SF2 is involved in the conversion of lanosterol to cholesterol and, specifically, catalyzes the NADPH dependant reduction of 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol to 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol and NADP+.
Immunogen:    Synthetic peptide of human TM7SF2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221029(TM7SF2 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221029(Anti-TM7SF2 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human fetal brain tissue;
Primary antibody: 221029(TM7SF2 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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