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TMED10 Rabbit pAb

Anti-TMED10 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219270

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219270
Product Name:   Anti-TMED10 Rabbit Polyclonal Antibody
Synonyms:   p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)
UNIPROT ID:   P49755 (Gene Accession – BC001496 )
Background:    This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex’s gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer’s disease. This gene has a pseudogene on chromosome 8.
Immunogen:    Fusion protein of human TMED10
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 219270(TMED10 Antibody) at a dilution of 1/65(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 219270(Anti-TMED10 Antibody) at dilution 1/65.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 219270(Anti-TMED10 Antibody) at a dilution of 1/65.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D226161(Anti-TMED10 Antibody) at dilution 1/65.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: NIH/3T3 cell, Mouse Pancreas tissue, Rat lung tissue, Mouse liver tissue, Hela, Raji cell lysates;
Primary antibody: 219270(TMED10 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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