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TOP1 Rabbit pAb

Anti-TOP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222309

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222309
Product Name:   Anti-TOP1 Rabbit Polyclonal Antibody
Synonyms:   TOPI
UNIPROT ID:   P11387 (Gene Accession – NP_003277 )
Background:    This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Immunogen:    Synthetic peptide of human TOP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human gastric cancer tissue using 222309(TOP1 Antibody) at a dilution of 1/35(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with the synthetic peptide and then with 222309(Anti-TOP1 Antibody) at dilution 1/35.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 and K562 cell lysates;
Primary antibody: 222309(TOP1 Antibody) at dilution 1/900;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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