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Topoisomerase I Rabbit mAb

Anti-Topoisomerase I Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N263009

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N263009
Product Name:   Anti-Topoisomerase I Rabbit Monoclonal Antibody
Synonyms:   TOP1; DNA topoisomerase 1; DNA topoisomerase I
UNIPROT ID:   P11387
Background:    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand.
Immunogen:    A synthetic peptide of human Topoisomerase I
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-2H9
MW:    Calculated MW: 91 kDa; Observed MW: 91 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Topoisomerase I(green) in Hela using Topoisomerase I antibody,and DAPI(blue)

Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Topoisomerase I antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
          Publications