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TPSB2 Rabbit pAb

Anti-TPSB2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217932

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217932
Product Name:   Anti-TPSB2 Rabbit Polyclonal Antibody
Synonyms:   TPS2; tryptaseB; tryptaseC
UNIPROT ID:   P20231 (Gene Accession – BC029356 )
Background:    Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3′ UTR and contain tandem repeat sequences at the 5′ flank and 3′ UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Immunogen:    Fusion protein of human TPSB2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 217932(TPSB2 Antibody) at a dilution of 1/140(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 217932(Anti-TPSB2 Antibody) at dilution 1/140.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human left side lung cancer tissue lysate;
Primary antibody: 217932(TPSB2 Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 4 minutes
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