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TRAP1 Rabbit pAb

Anti-TRAP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216565

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216565
Product Name:   Anti-TRAP1 Rabbit Polyclonal Antibody
Synonyms:   HSP75, HSP90L
UNIPROT ID:   Q12931 (Gene Accession – BC018950 )
Background:    HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol and endoplasmic reticulum
Immunogen:    Fusion protein of human TRAP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 216565(TRAP1 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the fusion protein and then with 216565(Anti-TRAP1 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 216565(Anti-TRAP1 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D220795(Anti-TRAP1 Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane 1-3: K562 cells, Jurkat cells, 293T cells;
Primary antibody: 216565(TRAP1 Antibody) at dilution 1/1050;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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