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TRIP4 Rabbit pAb

Anti-TRIP4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217171

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217171
Product Name:   Anti-TRIP4 Rabbit Polyclonal Antibody
Synonyms:   ASC1; ASC-1; HsT17391
UNIPROT ID:   Q15650 (Gene Accession – BC012448 )
Background:    Activating signal co-integrator-1 (ASC-1), originally identified as TRIP4, is a transcriptional co-activator of nuclear receptors that associates with specific components of the RNA polymerase II complex and binds the basal transcription factors TBP and TFIIA. ASC-1 functions with the transcription integrators SRC-1 and CBP/p300 through its zinc finger motif and is dependent on their ligand-dependent transactivation domain, AF2. Endogenous ASC-1 in HeLa cells is predominantly a nuclear protein. Under conditions of serum starvation, ASC-1 localizes to the cytoplasm.
Immunogen:    Fusion protein of human TRIP4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217171(TRIP4 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217171(Anti-TRIP4 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 217171(Anti-TRIP4 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D221914(Anti-TRIP4 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: A431 cells;
Primary antibody: 217171(TRIP4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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