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U2AF1 Rabbit pAb

Anti-U2AF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218300

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218300
Product Name:   Anti-U2AF1 Rabbit Polyclonal Antibody
Synonyms:   RN; FP793; U2AF35; U2AFBP; RNU2AF1
UNIPROT ID:   Q01081 (Gene Accession – BC001177 )
Background:    This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Immunogen:    Fusion protein of human U2AF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218300(U2AF1 Antibody) at a dilution of 1/130(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218300(Anti-U2AF1 Antibody) at dilution 1/130.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: 231 and HepG2 cell lysates;
Primary antibody: 218300(U2AF1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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