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UBA5 Rabbit pAb

Anti-UBA5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218303

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S218303
Product Name:   Anti-UBA5 Rabbit Polyclonal Antibody
Synonyms:   THIFP1; UBE1DC1
UNIPROT ID:   Q9GZZ9 (Gene Accession – BC009737 )
Background:    This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified.
Immunogen:    Fusion protein of human UBA5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218303(UBA5 Antibody) at a dilution of 1/30(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218303(Anti-UBA5 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 218303(Anti-UBA5 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with fusion protein and then with D224127(Anti-UBA5 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A431, HEPG2, Hela and 293T cell lysates;
Primary antibody: 218303(UBA5 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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