Email: info@neweastbio.com   |  Call: 6109452007

UBE2D1 Rabbit pAb

Anti-UBE2D1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218314

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218314
Product Name:   Anti-UBE2D1 Rabbit Polyclonal Antibody
Synonyms:   SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1
UNIPROT ID:   P51668 (Gene Accession – BC005980 )
Background:    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human UBE2D1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 200-400;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218314(UBE2D1 Antibody) at a dilution of 1/240(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218314(Anti-UBE2D1 Antibody) at dilution 1/240.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Jurkat, 231 and LOVO cell lysates;
Primary antibody: 218314(UBE2D1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
          Publications