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UBE2D3 Rabbit pAb

Anti-UBE2D3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218309

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218309
Product Name:   Anti-UBE2D3 Rabbit Polyclonal Antibody
Synonyms:   UBC4/5; UBCH5C; E2(17)KB3
UNIPROT ID:   P61077 (Gene Accession – BC003395 )
Background:    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 218309(UBE2D3 Antibody) at a dilution of 1/35(Nucleus and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the fusion protein and then with 218309(Anti-UBE2D3 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 218309(Anti-UBE2D3 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with fusion protein and then with D224134(Anti-UBE2D3 Antibody) at dilution 1/35.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: NIH/3T3 cell lysate;
Primary antibody: 218309(UBE2D3 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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