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UBE2O Rabbit pAb

Anti-UBE2O Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219477

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219477
Product Name:   Anti-UBE2O Rabbit Polyclonal Antibody
Synonyms:   E2-230K
UNIPROT ID:   Q9C0C9 (Gene Accession – BC022237 )
Background:    E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate ‘Lys-63’-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
Immunogen:    Fusion protein of human UBE2O
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 100-300; ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 219477(UBE2O Antibody) at a dilution of 1/120(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 219477(Anti-UBE2O Antibody) at dilution 1/120.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 219477(Anti-UBE2O Antibody) at a dilution of 1/120.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with fusion protein and then with D227298(Anti-UBE2O Antibody) at dilution 1/120.
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