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UCHL3 Rabbit pAb

Anti-UCHL3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217974

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217974
Product Name:   Anti-UCHL3 Rabbit Polyclonal Antibody
Synonyms:   UCH-L3
UNIPROT ID:   P15374 (Gene Accession – BC018125 )
Background:    The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 217974(UCHL3 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 217974(Anti-UCHL3 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 217974(Anti-UCHL3 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D223472(Anti-UCHL3 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T, K562, Jurkat and 231 cell;
Primary antibody: 217974(UCHL3 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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