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USP12 Rabbit pAb

Anti-USP12 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218326

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218326
Product Name:   Anti-USP12 Rabbit Polyclonal Antibody
Synonyms:   UBH1; USP12L1
UNIPROT ID:   O75317 (Gene Accession – BC026072 )
Background:    The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP12 (ubiquitin specific peptidase 12), also known as UBH1 or USP12L1, is a 370 amino acid protein belonging to the peptidase C19 family and the USP12/USP46 subfamily. Considered a deubiquitinating enzyme, it is suggested that USP12 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity. The gene encoding USP12 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218326(USP12 Antibody) at a dilution of 1/35(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218326(Anti-USP12 Antibody) at dilution 1/35.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse liver tissue lysate;
Primary antibody: 218326(USP12 Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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