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VCP Rabbit pAb

Anti-VCP Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218289

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218289
Product Name:   Anti-VCP Rabbit Polyclonal Antibody
Synonyms:   p97; TERA; ALS14; IBMPFD; HEL-220; IBMPFD1; HEL-S-70
UNIPROT ID:   P55072 (Gene Accession – BC121794 )
Background:    The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Immunogen:    Fusion protein of human VCP
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218289(VCP Antibody) at a dilution of 1/30(Nucleus and Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218289(Anti-VCP Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218289(Anti-VCP Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D224099(Anti-VCP Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: 231, Hela, A549, A431, HEPG2, Jurkat and K562 cell lysates;
Primary antibody: 218289(VCP Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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