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VMP1 Rabbit pAb

Anti-VMP1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221033

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221033
Product Name:   Anti-VMP1 Rabbit Polyclonal Antibody
Synonyms:   EPG3; TANGO5; TMEM49
UNIPROT ID:   Q96GC9 (Gene Accession – NP_112200 )
Background:    This gene encodes a transmembrane protein that plays a key regulatory role in the process of autophagy. The ectopic overexpression of the encoded protein in cultured cells triggers autophagy even under nutrient-rich conditions. This gene is overexpressed in pancreatitis affected acinar cells where the encoded protein mediates sequestration and degradation of potentially deleterious activated zymogen granules in a process termed, zymophagy.
Immunogen:    Synthetic peptide of human VMP1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer,Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221033(VMP1 Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221033(Anti-VMP1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221033(Anti-VMP1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D262380(Anti-VMP1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: MCF7 cell lysate;
Primary antibody: 221033(VMP1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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