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VPS35 Rabbit pAb

Anti-VPS35 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221567

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221567
Product Name:   Anti-VPS35 Rabbit Polyclonal Antibody
Synonyms:   MEM3; PARK17
UNIPROT ID:   Q96QK1 (Gene Accession – NP_060676 )
Background:    This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric comple,x termed the retromer comple,x involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Immunogen:    Synthetic peptide of human VPS35
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 221567(VPS35 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 221567(Anti-VPS35 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 221567(Anti-VPS35 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D263212(Anti-VPS35 Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: K562, MCF-7, Raji, HepG2, Hela, A549, Mouse fetal brain tissue lysates;
Primary antibody: 221567(VPS35 Antibody) at dilution 1/700;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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