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VWA5A Rabbit pAb

Anti-VWA5A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217205

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217205
Product Name:   Anti-VWA5A Rabbit Polyclonal Antibody
Synonyms:   BCSC1; BCSC-1; LOH11CR2A
UNIPROT ID:   O00534 (Gene Accession – BC001234 )
Background:    VWA5A (von Willebrand factor A domain containing 5A), also known as BCSC-1 (breast cancer suppressor candidate 1) or LOH11CR2A (loss of heterozygosity 11 chromosomal region 2 gene A protein), is a 786 amino acid protein containing one VIT domain and one VWFA domain. VWA5A is expressed at low levels in various tissues, with no expression found in 80% of tumor cell lines. Likely acting as a tumor suppressor gene, deletion of VWA5A leads to loss of heterozygosity (LOH) in breast and ovarian tumors, and may have an important role as a potential gene therapy target. Abnormal expression of VWA5A may lead to an increase in adhesion of CNE-2L2 cells associated with an increase in expression of E-cadherin, alpha-catenin, and p53, resulting in a decrease of malignant activity in cells with ectopic expression of VWA5A.
Immunogen:    Fusion protein of human VWA5A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217205(VWA5A Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217205(Anti-VWA5A Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 217205(Anti-VWA5A Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D221980(Anti-VWA5A Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Human Adrenal pheochromocytoma tissue, Jurkat cells, A549 cells;
Primary antibody: 217205(VWA5A Antibody) at dilution 1/275;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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