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WARS2 Rabbit pAb

Anti-WARS2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218277

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S218277
Product Name:   Anti-WARS2 Rabbit Polyclonal Antibody
Synonyms:   TrpRS; NEMMLAS; mtTrpRS
UNIPROT ID:   Q9UGM6 (Gene Accession – BC044575 )
Background:    Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.
Immunogen:    Fusion protein of human WARS2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218277(WARS2 Antibody) at a dilution of 1/120(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218277(Anti-WARS2 Antibody) at dilution 1/120.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: LNCAP, Hela, 231 cell lysates;
Primary antibody: 218277(WARS2 Antibody) at dilution 1/1600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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