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WASF1 Rabbit pAb

Anti-WASF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218278

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S218278
Product Name:   Anti-WASF1 Rabbit Polyclonal Antibody
Synonyms:   WAVE; SCAR1; WAVE1; NEDALVS
UNIPROT ID:   Q92558 (Gene Accession – BC044591 )
Background:    The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Immunogen:    Fusion protein of human WASF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 218278(WASF1 Antibody) at a dilution of 1/80(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 218278(Anti-WASF1 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: U251, Hela, NIH/3T3, K562, 293T cell lysates;
Primary antibody: 218278(WASF1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
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