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WASF3 Rabbit pAb

Anti-WASF3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221112

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S221112
Product Name:   Anti-WASF3 Rabbit Polyclonal Antibody
Synonyms:   SCAR3; WAVE3; Brush-1
UNIPROT ID:   Q9UPY6 (Gene Accession – NP_006637 )
Background:    This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants.
Immunogen:    Synthetic peptide of human WASF3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 221112(WASF3 Antibody) at a dilution of 1/25(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 221112(Anti-WASF3 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 221112(Anti-WASF3 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D262489(Anti-WASF3 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: A172, 293T and Hepg2 cell lysates;
Primary antibody: 221112(WASF3 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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