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WDFY2 Rabbit pAb

Anti-WDFY2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218284

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S218284
Product Name:   Anti-WDFY2 Rabbit Polyclonal Antibody
Synonyms:   PROF; WDF2; ZFYVE22
UNIPROT ID:   Q96P53 (Gene Accession – BC014004 )
Background:    WD repeat and FYVE domain-containing protein 2 (WDFY2), also known as WDF2 and ZFYVE22, is a 400 amino acid protein that localizes to a set of small endosomes that are found within 100 nm from the plasma membrane. Highly conserved between species, WDFY2 consists of one FYVE-type zinc finger and seven WD repeats. The FYVE domain is a cysteine-rich domain of about 70 amino acids. Its primary role is to target signal-transducing proteins to cell membranes through binding to the membrane lipid phosphatidylinositol-3-phosphate with high specificity. WD-repeats are generally found in clusters of seven. They have no intrinsic catalytic activity, but they serve as a platform for protein-protein interactions. WDFY2 is suspected to play a critical role in the endocytic pathway.
Immunogen:    Fusion protein of human WDFY2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 30-150;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 218284(WDFY2 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 218284(Anti-WDFY2 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse kidney tissue lysate;
Primary antibody: 218284(WDFY2 Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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