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WDFY3 Rabbit pAb

Anti-WDFY3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218281

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S218281
Product Name:   Anti-WDFY3 Rabbit Polyclonal Antibody
Synonyms:   ALFY; ZFYVE25
UNIPROT ID:   Q8IZQ1 (Gene Accession – BC015214 )
Background:    This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.
Immunogen:    Fusion protein of human WDFY3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218281(WDFY3 Antibody) at a dilution of 1/70(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218281(Anti-WDFY3 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218281(Anti-WDFY3 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224087(Anti-WDFY3 Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 218281(WDFY3 Antibody) at dilution 1/650;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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