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WEE2 Rabbit pAb

Anti-WEE2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221546

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221546
Product Name:   Anti-WEE2 Rabbit Polyclonal Antibody
Synonyms:   OOMD5; WEE1B
UNIPROT ID:   P0C1S8 (Gene Accession – NP_001099028 )
Background:    Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II (PubMed:29606300). Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at ‘Tyr-15’, leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at ‘Tyr-15’, to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).
Immunogen:    Synthetic peptide of human WEE2
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 25-100; ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221546(WEE2 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221546(Anti-WEE2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 221546(Anti-WEE2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D263187(Anti-WEE2 Antibody) at dilution 1/40.
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