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WIF1 Rabbit pAb

Anti-WIF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218031

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218031
Product Name:   Anti-WIF1 Rabbit Polyclonal Antibody
Synonyms:   WIF-1
UNIPROT ID:   Q9Y5W5 (Gene Accession – BC018037 )
Background:    The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Immunogen:    Fusion protein of human WIF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 218031(WIF1 Antibody) at a dilution of 1/30(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 218031(Anti-WIF1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218031(Anti-WIF1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D223575(Anti-WIF1 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Hela cell lysate;
Primary antibody: 218031(WIF1 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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