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WIPI2 Rabbit pAb

Anti-WIPI2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221555

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221555
Product Name:   Anti-WIPI2 Rabbit Polyclonal Antibody
Synonyms:   Atg21; ATG18B; CGI-50; WIPI-2
UNIPROT ID:   Q9Y4P8 (Gene Accession – NP_056425 )
Background:    WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids.
Immunogen:    Synthetic peptide of human WIPI2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221555(WIPI2 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221555(Anti-WIPI2 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 221555(Anti-WIPI2 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D263199(Anti-WIPI2 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human heart tissue lysate;
Primary antibody: 221555(WIPI2 Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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