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WNT1 Rabbit pAb

Anti-WNT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220302

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220302
Product Name:   Anti-WNT1 Rabbit Polyclonal Antibody
Synonyms:   INT1; OI15; BMND16
UNIPROT ID:   P04628 (Gene Accession – NP_005421 )
Background:    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Immunogen:    Synthetic peptide of human WNT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 220302(WNT1 Antibody) at a dilution of 1/50(Secreted).

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: HT-29, LOVO, Hela cell lysates;
Primary antibody: 220302(WNT1 Antibody) at dilution 1/300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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