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YWHAG Rabbit pAb

Anti-YWHAG Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219936

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219936
Product Name:   Anti-YWHAG Rabbit Polyclonal Antibody
Synonyms:   14-3-3GAMMA
UNIPROT ID:   P61981 (Gene Accession – NP_036611 )
Background:    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Immunogen:    Synthetic peptide of human YWHAG
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 219936(YWHAG Antibody) at a dilution of 1/100(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 219936(Anti-YWHAG Antibody) at dilution 1/100.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Hela cells, 293T cells, Jurkat cells;
Primary antibody: 219936(YWHAG Antibody) at dilution 1/1800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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