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YWHAQ Rabbit pAb

Anti-YWHAQ Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219937

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219937
Product Name:   Anti-YWHAQ Rabbit Polyclonal Antibody
Synonyms:   1C5, HS1, 14-3-3
UNIPROT ID:   P27348 (Gene Accession – NP_006817 )
Background:    This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5′ UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Immunogen:    Synthetic peptide of human YWHAQ
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 219937(YWHAQ Antibody) at a dilution of 1/20(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 219937(Anti-YWHAQ Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 219937(Anti-YWHAQ Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D260654(Anti-YWHAQ Antibody) at dilution 1/20.

Gel: 10+12%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Mouse brain tissue, A549 cells, human lymphoma tissue, NIH/3T3 cells, human colon cancer tissue;
Primary antibody: 219937(YWHAQ Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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