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ZAP70 ? Rabbit pAb

Anti-ZAP70 ? Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213311

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213311
Product Name:   Anti-ZAP70 Rabbit Polyclonal Antibody
Synonyms:   SRK; STD; TZK; STCD; ZAP-70
UNIPROT ID:   P43403 (Gene Accession – NP_001070 )
Background:    This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human ZAP70
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 213311(ZAP70 Antibody) at a dilution of 1/50(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 213311(Anti-ZAP70 Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cells;
Primary antibody: 213311(ZAP70 Antibody) at dilution 1/1000;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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