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ZBTB10 Rabbit pAb

Anti-ZBTB10 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221505

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S221505
Product Name:   Anti-ZBTB10 Rabbit Polyclonal Antibody
Synonyms:   RINZF
UNIPROT ID:   Q96DT7 (Gene Accession – NP_001099009 )
Background:    RINZF, also known as ZBTB10 (zinc finger and BTB domain containing protein 10), is a 847 amino acid protein that contains one BTB/POZ domain and two C2H2-type zinc fingers. Localized to the nucleus, RINZF is believed to play a role in transcriptional regulation. Specifically, RINZF is capable of binding to the CACC element of the Gastrin promoter. In this regard, RINZF competes with Sp1 for CACC binding and interferes with Sp1 transactivation, thereby regulating Gastrin gene expression. The rat RINZF protein shares 98% homology with the human RINZF protein, suggesting that RINZF is a conserved protein. Due to alternative splicing events, two RINZF isoforms exist. In addition, RINZF may be phosphorylated by ATR or ATM upon DNA damage.
Immunogen:    Synthetic peptide of human ZBTB10
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221505(ZBTB10 Antibody) at a dilution of 1/100(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221505(Anti-ZBTB10 Antibody) at dilution 1/100.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221505(Anti-ZBTB10 Antibody) at a dilution of 1/100.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D263131(Anti-ZBTB10 Antibody) at dilution 1/100.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: Jurkat cell lysate;
Primary antibody: 221505(ZBTB10 Antibody) at dilution 1/800;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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