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ZBTB3 ? Rabbit pAb

Anti-ZBTB3 ? Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213316

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213316
Product Name:   Anti-ZBTB3 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q9H5J0 (Gene Accession – NP_079060 )
Background:    The BTB (Broad-Comple,x Tramtrack and Bric a brac) domain, also known as the POZ (POxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. ZBTB3 (zinc finger and BTB domain containing 3) is a 574 amino acid protein that contains one BTB (POZ) domain and two C2H2-type zinc fingers. Localized to the nucleus, ZBTB3 is thought to play a role in transcriptional regulation events. The gene encoding ZBTB3 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome.
Immunogen:    Synthetic peptide of human ZBTB3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213316(ZBTB3 Antibody) at a dilution of 1/50(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213316(Anti-ZBTB3 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cells;
Primary antibody: 213316(ZBTB3 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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