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ZNF131 Rabbit pAb

Anti-ZNF131 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218232

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218232
Product Name:   Anti-ZNF131 Rabbit Polyclonal Antibody
Synonyms:   ZBTB35; pHZ-10
UNIPROT ID:   P52739 (Gene Accession – BC035875 )
Background:    Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the krueppel C2H2-type zinc-finger protein family, ZNF131 (Zinc finger protein 131) is a 623 amino acid nuclear protein that contains one BTB (POZ) domain and six C2H2-type zinc fingers. With predominant expression found in brain, it is likely that ZNF131 plays a role as a transcription regulator during development and organogenesis of the adult central nervous system. ZNF131 also represses ER† (Estrogen receptor alpha)-mediated transactivation by interrupting ER† binding to the estrogen-response element. There are two isoforms of ZNF131 that are produced as a result of alternative splicing events.
Immunogen:    Fusion protein of human ZNF131
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 218232(ZNF131 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 218232(Anti-ZNF131 Antibody) at dilution 1/30.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human testis tissue and Rat brain tissue lysates, Jurkat and Hela cell lysates;
Primary antibody: 218232(ZNF131 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 15 seconds
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