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Arf1(Δ17Q71L) Protein

Arf1 Protein Δ17 Q71L mutant
5/5

$347.00

Cat.#:  10123

   Size:   10 μl

In Stock

            Product Description          

Arf1(Δ17Q71L) Mutant

Cat. #:  10123
Product Name:  Arf1 Protein Δ17 Q71L mutant
Synonyms:  ADP-ribosylation factor 1
Source:   Human recombinant, His6-tag
Expression Host Species:   E. coli
Molecular Weight:   21 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Arf1 is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport.
Amino Acid Sequence   (1-181, Δ17, Q71L)
MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD
VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM
NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Arf1 Δ17Q71L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining


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