NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
ATM(F858L)
Cat. #: 26141 |
Gene Symbol: ATM |
Description: Anti-ATM(F858L) Mouse Monoclonal Antibody |
Background: Ataxia telangiectasia mutated (ATM) is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. It phosphorylates several key proteins that initiate activation of the DNA damage checkpoint, leading to cell cycle arrest, DNA repair or apoptosis. Activity of ATM protein is under tight control, and mutation of ATM can cause disease such as Ataxia telangiectasia (AT) and cancers. |
Immunogen: A synthetic peptide from the internal region of ATM which includes the mutation of F858L, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes ATM(F858L), but not wild type ATM protein from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant ATM(F858L) and wild type proteins. Purified His-tagged ATM(F858L) protein (amino acids 2400-3056, lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-ATM(F858L) mouse monoclonal antibody (Cat. #26141). Immunofluorescence: Immunofluorescence of cells expressing ATM(F858L) protein with anti-ATM(F858L) antibody. HEK293T cells were transfected with pCDNA3-GFP-ATM (WT) plasmid (left column) or pCDNA3-GFP-ATM(F858L) plasmid (right column), then fixed and stained with Anti-ATM(F858L) monoclonal antibody (Cat. #26141). |