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B7-2 (DM84) Rabbit mAb

Anti-B7-2 (DM84) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28409

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28409
Product Name:   Anti-B7-2(DM84) Rabbit Monoclonal Antibody
Synonyms:   CD86; B7-2; B70; CD28LG2; LAB72; MGC34413
Description:    Anti-B7-2 antibody(DM84) Rabbit Monoclonal Antibody
Background:    This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells; and it is the ligand for two proteins at the cell surface of T cells; CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human B7-2
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2 protein, mFc-His tagged protein 11162 can bind Rabbit anti-B7-2 monoclonal antibody  (clone: DM84) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein  (A) and human B7-2  (B) were surface stained with Rabbit anti-B7-2 monoclonal antibody 1µg/ml  (clone: DM84) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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