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BCMA (DM4) Rabbit mAb

Anti-BCMA (DM4) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28164

   Size:   100 μg

In Stock

            Product Description          
Cat.#:   28164
Product Name:   Anti-BCMA Bispecific(DM4)
Synonyms:   TNFRSF17
Description:    Anti-BCMA bispecific antibody(DM4)
Background:    BiTE bispecific antibody is an Engineered fusion protein constructed from two single-chain variable fragments (scFvs) of different monoclonal antibodies. One of the scFvs will be constructed from an anti-CD3 monoclonal antibody; and the other scFv fragment which linked by a linker region will be made from an anti-Tumor cell specific monoclonal antibody.The B-cell maturation protein (BCMA or BCM) is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes; and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily; member 13b (TNFSF13B:TALL-1:BAFF); and to lead to NF-kappaB and MAPK8:JNK activation. This receptor also binds to various TRAF family members; and thus may transduce signals for cell survival and proliferation. [provided by RefSeq; Jul 2008]
Applications:    Flow Cyt; MM Tumor cell killing
Recommended Dilutions:    Flow Cyt 1:100; MMTumor cell killing
Host Species:    Rabbit
Isotype:    Rabbit scfv
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human BCMA
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. The basic principle of BiTE cell killing assay. The BiTE molecule can effectively bring T cells to tumor target cells and stimulate tumor cell killing activity of T cells.

Figure 2. A: The scheme of Anti-BCMA BiTE molecule. B: Tumor cell killing assay. NCI-H929 cells (stably transfected with luciferase), were incubated with freshly isolated human PBMC, and different concentration of BiTE antibodies constructed from rabbit Anti-Human BCMA/TNFRSF17 Clone DM4 (red line), or BB2121 originated huC11D5.3 clone (blue line) or a no BCMA binding clone as negative control (black line). After 48hrs incubation, the viable NCI-H929 tumor cells were measured by luciferase activity assay.
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