NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
BRaf Antibody
Cat. #: 26129 |
Size: 100 µL |
Gene Symbol: B-Raf |
Description: Anti-B-RAF Mouse Monoclonal Antibody |
Background: B-Raf belongs to the TKL Ser/Thr protein kinase family, RAF subfamily. It is involved in the transduction of mitogenic signals from the cell membrane to the nucleus, and may play a role in the postsynaptic responses of hippocampal neuron. B-Raf is located to nucleus and cytoplasm, and expressed in brain and testis. Immunogen: A synthetic peptide from the internal region of B-Raf, human origin. |
Immunogen: A synthetic peptide from the internal region of Braf (V600K), human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:1000-1:5000 WB 1:100-1:1000 IHC 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-B-Raf antibody recognizes B-Raf of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Purified His-tagged B-Raf protein (Cat. #10161) was blotted with anti-B-Raf monoclonal antibody (Cat. #26129). |
1. Thyroid, Parathyroid and Adrenal Glands Handbook of Practical Immunohistochemistry, 2015 |