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IL1B mAb (Canakinumab)

Anti-IL1B Monoclonal Antibody (CanakinuMonoclonal Antibody)?


Cat.#:  28114

   Size:   100 μg

In Stock

            Product Description          
Cat.#:   28114
Product Name:   Anti-IL1B(Canakinumab) Monoclonal Antibody
Synonyms:   IL-1 beta;IL1F2
Description:    Anti-IL1B(canakinumab ) Monoclonal Antibody
Background:    Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Homo sapiens
Isotype:    IgG1
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human IL1B
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/mL (100 µl/well) Human IL1B Protein, hFc Tag (11619) can bind Anti-IL1B Neutralizing antibody (28114) in a linear range of 0.61-156.25 ng/mL. In order to specifically detect 28114, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. Flow cytometry analysis under cell membrane permeable condition with 1 µg/mL Anti-IL1B (canakinumab biosimilar) mAb (28114) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).