| Cat.#: 28114 |
| Product Name: Anti-IL1B(Canakinumab) Monoclonal Antibody |
| Synonyms: IL-1 beta;IL1F2 |
| Description: Anti-IL1B(canakinumab ) Monoclonal Antibody |
| Background: Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Homo sapiens |
| Isotype: IgG1 |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human IL1B |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 1 µg/mL (100 µl/well) Human IL1B Protein, hFc Tag (11619) can bind Anti-IL1B Neutralizing antibody (28114) in a linear range of 0.61-156.25 ng/mL. In order to specifically detect 28114, mouse anti-human Fab-specific antibody was used as detection antibody. | 
Figure 2. Flow cytometry analysis under cell membrane permeable condition with 1 µg/mL Anti-IL1B (canakinumab biosimilar) mAb (28114) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |
|
