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β-Catenin pAb

Anti-β-catenin Rabbit Polyclonal Antibody
5/5

$219.00

Cat.#:  21282

   Size:   100 μl

In Stock

          Product Description          

β-Catenin Polyclonal Antibody

Cat. #:  21282
Gene Symbol:  CTNNB1, CTNNB
Description:   Anti-β-catenin Rabbit Polyclonal Antibody
Background:   Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen:    A synthetic peptide from the internal region of β-catenin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:2000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   IgG
Purity:    Purified from serum
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes β-catenin from vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing

Western blot:  

Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #21282).
Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.
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