$495.00
| Cat. #: 26169 |
| Gene Symbol: CTNNB1, CTNNB |
| Description: Anti-β-catenin(G38D) Mouse Monoclonal Antibody |
| Background: Beta-catenin(or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer. |
| Immunogen: A synthetic peptide from the internal region of β-catenin which includes the mutation of G38D, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes β-catenin(G38D), but not wild type β-catenin protein from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant β-catenin(G38D) and wild type proteins. Purified His-tagged β-catenin(G38D) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-β-catenin(G38D) mouse monoclonal antibody (Cat. #26169).
Immunofluorescence:
Immunofluorescence of cells expressing β-catenin proteins with anti-β-catenin(G38D) antibody. HEK293T cells were transfected with pCDNA3-GFP-CTNNB1 (WT) plasmid (left column) or pCDNA3-GFP-CTNNB1(G38D) plasmid (right column), then fixed and stained with anti-β-catenin(G38D) monoclonal antibody (Cat. #26169). |
