NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26166 |
Gene Symbol: CTNNB1, CTNNB |
Description: Anti-β-catenin(G48D) Mouse Monoclonal Antibody |
Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer. |
Immunogen: A synthetic peptide from the internal region of β-catenin which includes the mutation of G48D, human origin. |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes β-catenin (G48D), but not wild type β-catenin protein from vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant β-catenin (G48D) and wild type proteins. Purified His-tagged β-catenin (G48D) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti- β-catenin (G48D) mouse monoclonal antibody (Cat. #26166). Immunofluorescence: Immunofluorescence of cells expressing β-catenin proteins with anti- β-catenin (G48D) antibody. HEK293T cells were transfected with pCDNA3-GFP-β-catenin (WT) plasmid (left column) or pCDNA3-GFP-β-catenin (G48D) plasmid (right column), then fixed and stained with anti- β-catenin (G48D) monoclonal antibody (Cat. #26166). |