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CD22 (DM13) Rabbit mAb

Anti-CD22 (DM13) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28301

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28301
Product Name:   Anti-CD22(DM13) Rabbit Monoclonal Antibody
Synonyms:   SIGLEC-2; SIGLEC2
Description:    Anti-CD22 antibody(DM13) Rabbit Monoclonal Antibody
Background:    CD22 (CD22 Molecule) is a Protein Coding gene. Diseases associated with CD22 include Refractory Hematologic Cancer and Hairy Cell Leukemia. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and Hematopoietic cell lineage. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC2.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human CD22
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Expi 293 cell line transfected with irrelevant protein  (left) and human CD22  (right) were surface stained with Rabbit anti- CD22 monoclonal antibody 1µg/ml ( clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody ( clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 3. Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones.  A) Heavy chain and  B) Light chain.
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