| Cat.#: 28357 |
| Product Name: Anti-CD27(DM57) Rabbit Monoclonal Antibody |
| Synonyms: CD27; TNFRSF7; S152; T14; Tp55 |
| Description: Anti-CD27 antibody(DM57) Rabbit Monoclonal Antibody |
| Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70; and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8:JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA); a proapoptotic protein; can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human CD27 |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
 
Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1µg/ml ( clone: DM57) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody ( clone: DM57) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | 
Figure 3. Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | |
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