| Cat.#: 28371 |
| Product Name: Anti-CD28(DM64) Rabbit Monoclonal Antibody |
| Synonyms: CD28; Tp44 |
| Description: Anti-CD28 antibody(DM64) Rabbit Monoclonal Antibody |
| Background: The protein encoded by this gene is essential for T-cell proliferation and survival; cytokine production; and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human CD28 |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28 protein, mFc-His tagged protein 11142 can bind Rabbit anti-CD28 monoclonal antibody (clone: DM64) in a linear range of 1-100 ng/ml. | 
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1µg/ml ( clone: DM64) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CD28 monoclonal antibody ( clone: DM64) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | 
Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
|
